dentiometric scanner Search Results


dentiometric scanner  (Bio-Rad)
90
Bio-Rad dentiometric scanner
HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using <t>dentiometric</t> scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.
Dentiometric Scanner, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dentiometric scanner/product/Bio-Rad
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dentiometric scanner - by Bioz Stars, 2026-03
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tlc scanner camag tlc scanner iii  (Camag)
90
Camag tlc scanner camag tlc scanner iii
HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using <t>dentiometric</t> scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.
Tlc Scanner Camag Tlc Scanner Iii, supplied by Camag, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/tlc scanner camag tlc scanner iii/product/Camag
Average 90 stars, based on 1 article reviews
tlc scanner camag tlc scanner iii - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

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HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using dentiometric scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.

Journal: BMC Cancer

Article Title: Tumor suppressor micro RNA miR-145 and onco micro RNAs miR-21 and miR-222 expressions are differentially modulated by Hepatitis B virus X protein in malignant hepatocytes

doi: 10.1186/1471-2407-14-721

Figure Lengend Snippet: HBx modulated expressions of target proteins in transiently transfected and HBV producing malignant hepatocytes. (A) Western blot confirmed proteins PTEN, p27 and MAP3K (Raf 1) – targets of miR-21, miR-222 and miR-145 respectively were increased accordingly in HBx transfected HepG2 cells. Cells were transfected with 1 μg and 2 μg of HBx plasmid respectively or pCXN2 as a control plasmid. (B) Expressions of PTEN, p27 and MAP3K (Raf 1) proteins in 1.3 fold HBV transfected HepG2 cells. HepG2 cells were transfected with 1 μg pUC19-HBV or 1 μg pUC19 vector as a control. (C) Western blot exhibited differential expression of target proteins PTEN, p27 and MAP3K (Raf 1) in constitutively HBV producing HepG2.2.15 and control HepG2 cell line. Cells were collected for protein analysis 48 h after each transfection. β actin was taken as endogenous control. Protein bands were quantified using dentiometric scanner (Bio-Rad). Below are the graphical representation of intensity ratio between target proteins (PTEN, p27 and MAP3K (Raf 1)) and β actin in each lane.

Article Snippet: Proteins bands were quantified using Dentiometric scanner (Bio-Rad-GS-800, USA).

Techniques: Transfection, Western Blot, Plasmid Preparation, Expressing